HISTOCHEMICAL STUDY OF HEAVY METALS LOCALIZATION IN EMBRYOPHYTES TISSUES IN COURSE OF PHYTOEXTRACTION

The paper studies histochemical analysis methods and possibilities of using them to define metals localization in tissues and organs of plants. Heavy metals localizations in the plant body are of importance when studying their toxic effect and plant mechanisms of re-sistance. Different organs, tissues and even different cells of separate plant tissue accrete metals variously. Distribution of metals in the whole body may become strongly inhomogene-ous. The paper analyses distribution and peculiarities of copper and cadmium accretion by pod and soya bean tissues in the course of phytoextraction without any influence and under the influence of external physical fields (constant magnetic field, ultraviolet irradiation). The research specified that metals localization predominantly takes place in the plant roots which tissues function as a barrier (endodermis) and protect stems, leaves, and generative organs from pollutants. External physical fields had a favorable effect on the plants in the course of Cu and Cd phytoextraction out of the soil. The plants were of greater vitality, contained more moisture under the identical conditions, and thus had a bigger amount of cell electrolyte es-sential for biochemical behavior in the plant cells and tissues.

Key words: phytoextraction, localization, heavy metals, copper, cadmium, plant cell, external physical fields, magnetic field, ultraviolet irradiation

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